The Functional Genomics Facility is the Garvan’s and The
Kinghorn Cancer Centre (TKCC)’s latest addition. The facility allows
users to conduct unbiased screens for genes or drugs that regulate
various cell biological processes. It runs as a “research driven, staff
Essen IncuCyte Zoom The IncuCyte brings
together an efficient and thorough set of solutions for long-term,
live-cell imaging. This imaging method allows you to expand the ways you
document and understand cellular growth, behaviour, and morphology. In
addition, it provides an effective kinetic read-out solution for the
next generation of in vitro biological assays.
Thermo ArrayScan VTI We have access to a
Thermo ArrayScan VTI high content microscopic imager equipped with a
CatalystXpress Robot accessing a plate hotel and an automated Cytomat
(CO2 incubator). High content imaging uses fluorescence-based reagents,
advanced optical imaging instrumentation and sophisticated image
analysis software to quantitatively analyse physiological processes in
cells. A range of real time and end-point assays are feasible, including
cell number, proliferation, apoptosis, cell size, shape and viability
measures, cell migration and invasion.
Caliper Zephyr A Caliper Zephyr liquid handling robot is available for all transfections, liquid handling and other specialty applications.
Biotek EL406 equipped with a Biostack 3 Biotek EL406 and the Biostack3 allow users to aspirate and dispense media, cells, fixative, stains and drugs.
Garvan/TKCC users have access to the human ORFeome V8.1 (2011 release) library and high throughput cloning. A variety of expression vectors are also available.
For further information please contact Dr William Hughes at firstname.lastname@example.org.